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AP‐1 DNA‐binding activity is inhibited by selenite and selenodiglutathione
Author(s) -
Spyrou Giannis,
Björnstedt Mikael,
Kumar Sushil,
Holmgren Arne
Publication year - 1995
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(95)00599-5
Subject(s) - selenium , thioredoxin reductase , thioredoxin , dna , chemistry , microbiology and biotechnology , cell growth , biochemistry , transcription factor , binding site , biology , enzyme , gene , organic chemistry
The binding of the transcription factor AP‐1 to DNA has been shown to be modulated by redox control mechanisms. Selenite and selenodiglutathione (GS‐Se‐SG), inhibit mammalian cell growth and are efficient oxidants of reduced thioredoxin and reduced thioredoxin reductase. Here, we report that selenite and GS‐Se‐SG efficiently inhibited AP‐1 DNA‐binding in nuclear extracts from 3B6 lymphocytes. A GS‐Se‐SG concentration of 0.75 μM resulted in 50% inhibition of AP‐1 DNA‐binding, whereas the same effect was achieved with 7.5 μM selenite. Nuclear extracts prepared from human 3B6 lymphocytes exposed for 4 h to 10 μM selenite showed a 50% reduction of AP‐1 binding. These data suggest that selenite and selenodiglutathione inactivate the AP‐1 factor and provide a mechanism by which selenium compounds inhibit cell growth.