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Regulation of cell surface GLUT1, GLUT3, and GLUT4 by insulin and IGF‐I in L6 myotubes
Author(s) -
Wilson Cindy M.,
Mitsumoto Yasuhide,
Maher Fran,
Klip Amira
Publication year - 1995
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(95)00589-2
Subject(s) - glut4 , glut3 , myogenesis , glut1 , chemistry , glucose transporter , microbiology and biotechnology , medicine , insulin , endocrinology , myocyte , biology
The effects of insulin and IGF‐I on the cell surface quantities of GLUT1, GLUT3 and GLUT4 glucose transporters in L6 myotubes were determined with the exofacial bis‐mannose phololabel (ATB‐BMPA). In basal cells, an equal molar quantity of each transporter isoform was found at the cell surface. Insulin stimulated the translocation of all three glucose transporter isoforms to the plasma membrane fraction from the light microsome fraction, resulting in equal molar quantities on the cell surface. IGF‐I stimulated a similar translocation of all isoforms, augmented by an increase in surface GLUT3 as assessed by ATB‐BMPA.