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Structure/function studies on cytoskeletal proteins in Dictyostelium amoebae as a paradigm
Author(s) -
Schleicher Michael,
André Bernadette,
Andréoli Christophe,
Eichinger Ludwig,
Haugwitz Michael,
Hofmann Andreas,
Karakesisoglou Jacques,
Stöckelhuber Mechthild,
Noegel Angelika A.
Publication year - 1995
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(95)00579-x
Subject(s) - profilin , cytoskeleton , dictyostelium , actin , biology , microbiology and biotechnology , context (archaeology) , actin remodeling , actin binding protein , actin cytoskeleton , dictyostelium discoideum , microfilament , function (biology) , gene , cell , biochemistry , paleontology
The actin cytoskeleton in motile non‐muscle cells is being regulated by a large number of actin‐binding proteins. A deeper insight into the complex nature of the dynamic rearrangements of the microfilament system during cell movement requires an experimental system that allows the combined application of biochemical, biophysical, cell biological and molecular methods. Dictyostelium amoebae are well suited especially for a genetic approach because they are amenable to gene disruption, antisense and gene tagging techniques. The actin‐binding proteins profilin, hisactophilin and protovillin are being described in this context as typical examples that either bind to G‐actin, or anchor the actin cytoskeleton to the plasma membrane, or are structurally similar to vertebrate proteins but distinct in their functions.