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Intracellular transport of inositol‐containing sphingolipids in the yeast, Saccharomyces cerevisiae
Author(s) -
Hechtberger Petra,
Daum Günther
Publication year - 1995
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(95)00567-s
Subject(s) - golgi apparatus , sphingolipid , brefeldin a , microbiology and biotechnology , secretion , nocodazole , cycloheximide , saccharomyces cerevisiae , secretory pathway , yeast , inositol , intracellular , biology , chemistry , biochemistry , endoplasmic reticulum , cell , protein biosynthesis , receptor , cytoskeleton
Organelles of the early protein secretion pathway (ER, Golgi) are involved in biosynthesis and intracellular migration of the yeast sphingolipids, inositolphosphorylceramide (IPC), mannosylinositolphosphorylceramide (MIPC), and mannosyldiinositolphosphorylceramide (M(IP) 2 C). Cycloheximide and nocodazole neither block biosynthesis of sphingolipids, nor ER to Golgi transport of IPC. In contrast, treatment of yeast cells with brefeldin A, which affects integrity of the Golgi, decreases formation of IPC and MIPC. Interruption of late steps of protein secretion (Golgi to plasma membrane transport) in temperature‐sensitive secretory mutants prevents sphingolipids from being transported to the cell periphery.