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Green‐fluorescent protein mutants with altered fluorescence excitation spectra
Author(s) -
Ehrig Torsten,
O'Kane Dennis J.,
Prendergast Franklyn G.
Publication year - 1995
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(95)00557-p
Subject(s) - chromophore , fluorescence , green fluorescent protein , mutant , fluorescent protein , mutagenesis , aequorea victoria , biophysics , chemistry , site directed mutagenesis , excitation , mutation , photochemistry , biology , biochemistry , gene , physics , optics , quantum mechanics
Using random mutagenesis and visual selection of fluorescent clones, we have isolated a T2031 and a E222G mutant of the Aequorea green‐fluorescent protein. Each mutant has one of the two fluorescence excitation bands of the wild type deleted and retains the other without a wavelength shift. This finding is consistent with each excitation band corresponding to a distinct spectroscopic state of the chromophore. Both mutations are single amino acid exchanges which in the linear sequence are located remotely from the chromophore but in the folded protein may be situated in its vicinity. We conclude that the mutations influence the fluorescence properties by changing the interactions between the chromophore and its protein environment.