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Activation of a novel form of phospholipase A 2 during liver regeneration
Author(s) -
Wojtaszek Paul A.,
Van Putten Vicki,
Nemenoff Raphael A.
Publication year - 1995
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(95)00556-o
Subject(s) - liver regeneration , regeneration (biology) , stimulation , phospholipase , phospholipase a2 , enzyme , chemistry , microbiology and biotechnology , biochemistry , enzyme activator , fractionation , biology , endocrinology , chromatography
Activation of phospholipase A 2 (PLA 2 ) occurs following mitogenic stimulation of cells. This study examined PLA 2 activation during liver regeneration. Increased activity was detected within 1 h after partial hepatectomy, was maximal by 6 h, and returned to control levels by 24 h. Fractionation of cell‐free extracts revealed multiple peaks of PLA 2 activity. One peak appeared identical to the previously described cPLA 2 , and was modestly stimulated during regeneration. A higher molecular weight form (hPLA 2 ) was stimulated ∼5‐fold during regeneration. This enzyme was Ca 2+ ‐dependent and selective for arachidonoylphosphatidylethanolamine. The activation of this novel form of PLA 2 represents an early event in liver regeneration, and is likely to contribute to the proliferative response.