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Upregulation of interleukin‐8 receptor in human polymorphonuclear neutrophils by formyl peptide and lipopolysaccharide
Author(s) -
Manna Sunil K.,
Samanta Ajoy K.
Publication year - 1995
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(95)00525-e
Subject(s) - degranulation , receptor , downregulation and upregulation , lipopolysaccharide , interleukin 8 , cytokine , chemistry , immunology , interleukin , interleukin 1 receptor , pathogenesis , biology , biochemistry , gene
Interleukin‐8 (IL‐8) is implicated in the pathogenesis of a large number of neutrophil‐driven inflammatory diseases. Although the cytokine activates neutrophils through a receptor, no information is available regarding the regulation of IL‐8 receptor (IL‐8R) expression. The present study shows that, compared to control, the bacterial products — formylpeptide and LPS (serum‐activated) upregulate IL‐8 receptor by 54% and 115%, respectively, the former by degranulation of the secretory vesicle and the latter by de novo protein synthesis. The newly expressed IL‐8R could be demonstrated with anti‐IL‐8R‐antibody and by autoradiogram of the receptor crosslinked with [ 125 I]IL‐8. The study may be useful for understanding the potential role of IL‐8 during neutrophil mediated inflammatory response.