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Lack of correlation between mRNA expression and enzymatic activity of the aspartate aminotransferase isoenzymes in various tissues of the rat
Author(s) -
Abruzzese F.,
Greco M.,
Perlino E.,
Doonan S.,
Marra E.
Publication year - 1995
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(95)00518-e
Subject(s) - isozyme , enzyme , messenger rna , biochemistry , chemistry , microbiology and biotechnology , biology , gene
Little is known about control of expression of basal levels of the aspartate aminotransferases which are ubiquitous ‘house keeping’ enzymes in vertebrates. We have measured both mRNA and activity levels for both isoenzymes in various rat tissues as a function of age. Patterns of mRNA expression for the two isoenzymes were similar in a particular tissue about differed widely between tissues. Surprisingly, there was no simple correlation between mRNA levels and specific activities of the enzyme products. We conclude that translation for mRNA for these two isoenzymes is subject to tissue‐specific, and in some cases age‐related, regulation.