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Interleukin‐1β antagonizes phenobarbital induction of several major cytochromes P450 in adult rat hepatocytes in primary culture
Author(s) -
Abdel-Razzak Ziad,
Corcos Laurent,
Fautrel Alain,
Guillouzo André
Publication year - 1995
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(95)00513-9
Subject(s) - phenobarbital , cytochrome p450 , enzyme inducer , primary culture , basal (medicine) , chemistry , hepatocyte , cell culture , microbiology and biotechnology , medicine , endocrinology , biology , enzyme , biochemistry , in vitro , genetics , insulin
We have investigated the effects of interleukin (IL)‐1β and IL6 on expression and phenobarbital (PB) induction of ethoxyresorufin O ‐deethylase (EROD) and pentoxyresorufin O ‐deethylase (PROD) activities, as well as on mRNA levels of cytochromes P450 (CYP) 1A, 2B, 2C, 2E and 3A, in rat hepatocytes in primary culture. IL6 slightly antagonized PB‐induced PROD activity. Strikingly, IL1β strongly inhibited basal EROD and PROD activities, and fully blocked their induction by PB in a dose‐dependent fashion. Furthermore IL1β completely suppressed PB induction of all CYP mRNAs analyzed. Our results demonstrate that IL1β can suppress basal CYP activities, as well as PB‐inducible expression of five CYP mRNAs in rat hepatocytes in primary culture.