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The depolarization response element in acetylcholine receptor genes is a dual‐function E box
Author(s) -
Su Ching-Tien,
Huang Chang-Fen,
Schmidt Jakob
Publication year - 1995
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(95)00496-v
Subject(s) - depolarization , acetylcholine receptor , acetylcholine , biophysics , microbiology and biotechnology , chemistry , stimulation , receptor , biology , biochemistry , endocrinology
All acetylcholine receptor subunit genes contain E boxes and are blocked by membrane depolarization. We have used transfected C2C12 myogenic cells to investigate the response, to electrical stimulation and KCl, of wildtype and mutant regulatory regions of the chick acetylcholine receptor α, γ and δ subunit, and the mouse MLC genes. Point mutations revealed that E boxes function as activating elements targeted by the depolarization signal. These experiments suggest, and insertion of a depolarization response element into an unrelated promoter confirms, that plasma membrane depolarization switches the depolarization response element from an activating to a repressive mode.