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The nucleotide sequence of the bacteriophage T5 ltf gene
Author(s) -
Kaliman Alexander V.,
Kulshin Vladimir E.,
Shlyapnikov Michael G.,
Ksenzenko Vladimir N.,
Kryukov Valentine M.
Publication year - 1995
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(95)00482-o
Subject(s) - terminator (solar) , open reading frame , bacteriophage , gene , nucleic acid sequence , biology , coding region , genetics , peptide sequence , bamhi , microbiology and biotechnology , plasmid , escherichia coli , ionosphere , physics , astronomy
The nucleotide sequence of the bacteriophage T5 Bg /II‐ Bam HI fragment (4,835 bp in length) known to carry a gene encoding the LTF protein which forms the phage L‐shaped tail fibers was determined. It was shown to contain an open reading frame for 1,396 amino acid residues that corresponds to a protein of 147.8 kDa. The coding region of ltf gene is preceded by a typical Shine‐Dalgarno sequence. Downstream from the ltf gene there is a strong transcription terminator. Data bank analysis of the LTF protein sequence reveals 55.1% identity to the hypothetical protein ORF 401 of bacteriophage λ in a segment of 118 amino acids overlap.