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Co‐operative regulation of endocytosis by three RAB5 isoforms
Author(s) -
Bucci Cecilia,
Lütcke Anne,
Steele-Mortimer Olivia,
Olkkonen Vesa M.,
Dupree Paul,
Chiariello Mario,
Bruni Carmelo B.,
Simons Kai,
Zerial Marino
Publication year - 1995
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(95)00477-q
Subject(s) - endocytosis , rab , endocytic cycle , endosome , microbiology and biotechnology , gene isoform , gtpase , transferrin receptor , biology , transferrin , bulk endocytosis , receptor , biochemistry , intracellular , gene
Rab proteins are small GTPases involved in the regulation of membrane traffic. Rab5a has been shown to regulate transport in the early endocytic pathway. Here we report the isolation of cDNA clones encoding two highly related isoforms, Rab5b and Rab5c. The two proteins share with Rab5a all the structural features required for regulation of endocytosis. Rab5b and Rab5c colocalize with the both transferrin receptor and Rab5a, stimulate the homotypic fusion between early endosomes in vitro and increase the rate of endocytosis when overexpressed in vivo. These data demonstrate that three Rab5 isoforms cooperate in the regulation of endocytosis in eukaryotic cells.