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Studies on human porin: XIII. The type‐1 VDAC ‘Porin 31HL’ biotinylated at the plasmalemma of Trypan blue excluding human B lymphocytes
Author(s) -
Jakob Christian,
Götz Hilde,
Hellmann Thea,
Hellmann Klaus Peter,
Reymann Susanne,
Flörke Harald,
Thinnes Friedrich P.,
Hilschmann Norbert
Publication year - 1995
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(95)00465-l
Subject(s) - porin , voltage dependent anion channel , biotinylation , biochemistry , membrane protein , blot , bacterial outer membrane , chemistry , microbiology and biotechnology , biology , membrane , gene , escherichia coli
In 1989, we demonstrated for the first time the expression of the VDAC ‘Porin 31HL’ in the plasmalemma of human B lymphocytes, then giving first evidence of a multi‐topological expression of VDAC. Meanwhile, data from this and other laboratories support our proposal of a multi‐compartment distribution of the channel in mammalian tissues. Here, by biotinylation of plasmalemma‐integrated proteins of proven living B lymphocytes, followed by two‐dimensional electrophoresis, immuno‐ and streptavidin affinity blotting, we show that part of the channel molecules can be labelled at the outer membrane of the cells. Thus, by a relevant approach our results invalidate objections concerning putative cross‐reactivity of anti‐human Type‐1 porin antibodies with non‐VDAC proteins at the outer cell membrane.