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Almost the entire 5′ non‐translated region of hepatitis C virus is required for cap‐independent translation
Author(s) -
Rijnbrand René,
Bredenbeek Peter,
van der Straaten Tahar,
Whetter Linda,
Inchauspé Geneviève,
Lemon Stanley,
Spaan Willy
Publication year - 1995
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(95)00458-l
Subject(s) - translation (biology) , virology , hepatitis a virus , virus , biology , genetics , messenger rna , gene
To investigate which hairpin structures within the 5′ untranslated region of hepatitis C virus (HCV) are necessary for cap‐independent translation, mutants were constructed that lack one or more hairpin structures. Here we demonstrate, by constructing precisely defined hairpin deletion mutants, that with the exception of the most 5′ located hairpin structure, which on deletion shows an increase on translation, each of the predicted hairpins is found to be essential for cap‐independent translation. In addition, we demonstrate that HCV 5′UTR driven translation is stimulated by poliovirus 2A pro co‐expression.