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Regulation of the human spermidine synthase mRNA translation by its 5′‐untranslated region
Author(s) -
Kauppinen Leila
Publication year - 1995
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(95)00437-e
Subject(s) - spermidine , reticulocyte , messenger rna , microbiology and biotechnology , untranslated region , biology , atp synthase , translation (biology) , five prime untranslated region , three prime untranslated region , in vitro , enzyme , biochemistry , chemistry , gene
An increased mRNA content of spermidine synthase was found in phytohemagglutinin stimulated human peripheral lymphocytes and in cultured human myeloma (Sultan) cells stimulated to grow by change of the culture medium. The many‐fold increase in the amount of the message was accompanied by stimulation of the enzyme activity in activated lymphocytes, but not in stimulated myeloma cells. In the present study the effect of the 5′‐untranslated region of spermidine synthase mRNA on the post‐transcriptional control of its expression was studied both in vitro in rabbit reticulocyte system and in cultured mammalian cells. The results show that the GC‐rich 5′‐untranslated region of spermidine synthase mRNA has an inhibitory effect on its translation.