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Isolation and characterization of the cDNA for pea chloroplast SecA Evolutionary conservation of the bacterial‐type SecA‐dependent protein transport within chloroplasts
Author(s) -
Nohara Tetsuya,
Nakai Masato,
Goto Akira,
Endo Toshiya
Publication year - 1995
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(95)00415-6
Subject(s) - chloroplast , isolation (microbiology) , complementary dna , chemistry , biology , microbiology and biotechnology , biochemistry , gene
We report here the isolation of the cDNA for pea chloroplast SecA. Pea SecA encodes a polypeptide of 1,011 amino acids and shows high sequence similarity with cyanobacterial SecA. Pea SecA was synthesized as a larger precursor and was imported into isolated chloroplasts in vitro. The purified pea SecA, which was expressed in Escherichia coli cells, stimulated the in vitro import of the 33 kDa protein of the oxygen‐evolving complex into thylakoids. These results indicate that higher plant chloroplasts contain a bacterial‐type SecA protein‐dependent system for the intraorganellar protein transport into thylakoids.