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Phosphatidylcholine‐phospholipase C mediates the induction of nerve growth factor in cultured glial cells
Author(s) -
Laviada Inés D.,
Baudet Cristel,
Galve-Roperh Ismael,
Naveilhan Philippe,
Brachet Philippe
Publication year - 1995
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(95)00414-5
Subject(s) - phosphatidylcholine , nerve growth factor , phospholipase c , chemistry , microbiology and biotechnology , phospholipase , biophysics , biology , biochemistry , phospholipid , signal transduction , membrane , enzyme , receptor
Addition of phosphatidylcholine‐hydrolyzing phospholipase C (PC‐PLC) to cultured glial cells increased the levels of nerve growth factor (NGF) mRNA and the amount of cell‐secreted NGF. The effect of PC‐PLC was 2.5 times higher than that elicited by 4β‐phorbol 12β‐myristate 13α‐acetate. In cells in which protein kinase C (PKC) was fully inhibited or downregulated, the effect of PC‐PLC was reduced ‐ though still evident ‐ and similar to that exerted by sphingosine. Results thus indicate that PC‐PLC induces the synthesis of NGF by glial cells by a PKC‐dependent and PKC‐independent mechanisms.