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The gene coding for the mustard trypsin inhibitor‐2 is discontinuous and wound‐inducible
Author(s) -
Luigi R. Ceci,
N. Spoto,
Maddalena de Virgilio,
Raffaele Gallerani
Publication year - 1995
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(95)00386-n
Subject(s) - gene , coding region , intron , genomic dna , trypsin inhibitor , microbiology and biotechnology , gene expression , biology , complementary dna , genetics , northern blot , trypsin , biochemistry , enzyme
The gene coding for the mustard trypsin inhibitor‐2 has been isolated from a genomic library and characterized. Comparison of genomic and cDNA sequences indicates that the gene is interrupted by an intron of 193 bp. The eukaryotic peculiar regulatory sequences have been detected in the 5′ flanking region of the gene. In addition, a decanucleotide has been detected that is highly similar to the proposed G‐☐ and to the ABRE motifs required for the gene expression induced by methyl jasmonate and abscissic acid. Northern blot analysis demonstrates that the gene is expressed in immature seeds as well as in wounded leaves.