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Substrate interactions in cytochrome P‐450: correlation between carbon‐13 nuclear magnetic resonance chemical shifts and C‐O vibrational frequencies
Author(s) -
Nathalie Legrand,
Arnaud Bondon,
Gérard Simonneaux,
Christiane Jung,
E. W. Gill
Publication year - 1995
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(95)00377-l
Subject(s) - chemical shift , steric effects , chemistry , camphor , ligand (biochemistry) , cytochrome , substrate (aquarium) , resonance (particle physics) , nuclear magnetic resonance , stereochemistry , linear correlation , crystallography , organic chemistry , enzyme , biochemistry , receptor , physics , biology , ecology , statistics , mathematics , particle physics
13 CO NMR chemical shifts and 12 CO infrared stretching frequencies have been measured for cytochrome P‐450camCO in the presence of d ‐camphor and of various camphor analogues. A linear correlation between both parametersδ( 13 C and ν(CO) was found indicating that the steric and electrostatic interactions acting on the CO ligand are influenced by the substrate. It has been proved that P‐450 complexes are on another line in this correlation than hemoglobins which is explained by the different proximal ligand.