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Homologues of the engrailed gene from five molluscan classes
Author(s) -
Wray C.G.,
Jacobs D.K.,
Kostriken R.,
Vogler A.P.,
Baker R.,
DeSalle R.
Publication year - 1995
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(95)00372-g
Subject(s) - engrailed , homeobox , biology , homology (biology) , gene , genetics , polyplacophora , evolutionary biology , nucleic acid sequence , drosophila melanogaster , gene expression , zoology , mollusca
We used the polymerase chain reaction (PCR) to amplify, clone, and sequence 10 engrailed homeodomains from 8 species in the five major molluscan classes, including the serially organized chiton (Polyplacophora) lineage. The Drosophila melanogaster gene engrailed ( en ) is one of several genes involved in embyonic segment polarity determination. Studies of engrailed sequence and expression in molluscs are of interest due to questions regarding the evolution and homology of segmentation in these taxa. Nucleotide and deduced amino acid sequence comparisons reflect evolutionary conservation within helices of the en homeodomain and ancient divergences in the region 3′ to the homeodomain.