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DARPP‐32 (dopamine and cAMP‐regulated phosphoprotein, M r 32,000) is a membrane protein in the bovine parathyroid
Author(s) -
Matovcik Lisa M.,
Hemmings Hugh C.,
Kinder Barbara K.
Publication year - 1995
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(95)00359-h
Subject(s) - phosphoprotein , cytoplasm , chemistry , phosphatase , biochemistry , microbiology and biotechnology , endocrinology , medicine , biology , enzyme , phosphorylation
A distinct form of DARPP‐32, a protein phosphatase‐1 inhibitor, has been identified in bovine calf parathyroid glands. Immunoblot analysis of parathyroid tissue revealed a 32 kDa protein present predominantly in a particulate fraction; it remained particulate after treatment with 1.0 M NaCl or 0.1 M Na 2 CO 3 . Metabolic labeling of parathyroid cells with mevalonolactone demonstrated that DARPP‐32 is isoprenylated. Immunocytochemical localization studies demonstrated that DARPP‐32 is present in vesicles throughout the cytoplasm of parathyroid cells, and that protein phosphatase‐1γ is concentrated in the region of the plasma membrane. Thus, in contrast to the predominately soluble form of DARPP‐32 that has been characterized in selected areas of the central nervous system, the parathyroid form is tightly associated with intracellular membranes.