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Cloning, functional expression and tissue distribution of human α 1C ‐adrenoceptor splice variants
Author(s) -
Hirasawa Akira,
Shibata Katsushi,
Horie Kuniko,
Takei Yoshinori,
Obika Kenji,
Tanaka Teruo,
Muramoto Noriyuki,
Takagaki Kazuchika,
Yano Junichi,
Tsujimoto Gozoh
Publication year - 1995
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(95)00330-c
Subject(s) - gene isoform , alternative splicing , splice , cloning (programming) , complementary dna , chinese hamster ovary cell , microbiology and biotechnology , biology , clone (java method) , alpha (finance) , transfection , molecular cloning , rna splicing , interleukin 10 receptor, alpha subunit , receptor , gene , genetics , g alpha subunit , protein subunit , rna , medicine , construct validity , nursing , computer science , patient satisfaction , programming language
We report the cloning and characterization of two isoforms of human α 1C ‐adrenoceptor cDNA ( α 1C ‐2, α C ‐3). These isoforms are generated by alternative splicing and differ from the clone we previously isolated ( α 1C ‐1) in their length and sequences of the C‐terminal domain. Tissue distribution of mRNAs showed that these variants co‐express with α 1C ‐1 in the human heart, liver, cerebellum and cerebrum. Despite the structural differences, functional experiments in transfected CHO cells showed that the three isoforms have similar ligand binding properties, and all couple with phospholipase C/Ca 2+ signaling pathway.