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The transcriptional regulation of human arachidonate 12‐lipoxygenase gene by NFκB/Rel
Author(s) -
Arakawa Toshiya,
Nakamura Michihiro,
Yoshimoto Tanihiro,
Yamamoto Shozo
Publication year - 1995
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(95)00293-i
Subject(s) - p50 , gene , microbiology and biotechnology , nf κb , luciferase , chemistry , footprinting , gene expression , dna footprinting , lipoxygenase , biology , promoter , transcription factor , enzyme , biochemistry , transfection , signal transduction
As examined by the luciferase assay, a negative regulatory region including the NFκB motif was found in the 5′‐flanking region of the 12‐lipoxygenase gene in human erythroleukemia cells. The negative control was abolished by a site‐specific mutation of the NFκB motif. Probes including the NFκB region gave positive bands upon a gel‐shift assay. The bands were supershifted by antibodies for NFκB p50, NFκB p65 and c‐Rel, and were lost by a NFκB competitor DNA. Furthermore, the NFκB sequence was protected in DNase I footprinting. Thus, two kinds of heterodimer (p50 and p65; p50 and c‐Rel) seemed to control the over‐expression of the human 12‐lipoxygenase gene.