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High expression of a novel carnitine palmitoyltransferase I like protein in rat brown adipose tissue and heart: isolation and characterization of its cDNA clone
Author(s) -
Yamazaki Naoshi,
Shinohara Yasuo,
Shima Atsushi,
Terada Hiroshi
Publication year - 1995
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(95)00277-g
Subject(s) - complementary dna , brown adipose tissue , carnitine palmitoyltransferase i , cdna library , microbiology and biotechnology , biology , white adipose tissue , clone (java method) , carnitine , adipose tissue , homology (biology) , open reading frame , peptide sequence , amino acid , biochemistry , gene , beta oxidation , enzyme
To characterize energy metabolism in rat brown adipose tissue (BAT), we carried out differential screening of a cDNA library of BAT with a cDNA probe of white adipose tissue (WAT) and isolated one cDNA clone. It contained a single open reading frame of 2,316 bases which encodes a protein of 88.2 kDa. The predicted amino acid sequence showed the highest homology (62.6%) with that of rat carnitine palmitoyltransferase I (CPTI). The transcript corresponding to this cDNA was found to be abundantly expressed in BAT and heart. Therefore, the isolated clone is concluded to encode a CPTI like protein expressed in BAT and heart.