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Human CuZn superoxide dismutase enzymatic activity in cells is regulated by the length of the mRNA
Author(s) -
Kilk Ann,
Laan Maris,
Torp Aivar
Publication year - 1995
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(95)00266-c
Subject(s) - polyadenylation , microbiology and biotechnology , messenger rna , complementary dna , three prime untranslated region , untranslated region , biology , superoxide dismutase , gene , gene expression , plasmid , enzyme , genetics , biochemistry
Single functional human CuZnSOD gene encodes two species of mRNA differing in size by 200 nucleotides in the 3′‐untranslated region (UTR). We studied the expression of the CuZnSOD cDNA with different 3′‐ and 5′‐UTR. Deletion in the 5′‐end does not affect the expression of the enzyme, however, deletion in the 3′‐UTR decreases the level of expression of CuZnSOD. The plasmids containing the long CuZnSOD cDNA with all polyadenylation signal sequences utilize primarily the last polyadenylation site and give a long mRNA, which produces three times more enzyme than the short mRNA lacking the last polyadenylation site and the AU‐rich region.