Premium
Inositol(1,3,4,5) tetrakisphosphate plays an important role in calcium mobilization from Entamoeba histolytica
Author(s) -
Raha Sanghamitra,
Giri Banabihari,
Bhattacharyya Bhudeb,
Biswas Birendra B
Publication year - 1995
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(95)00265-b
Subject(s) - entamoeba histolytica , calcium , inositol , inositol phosphate , chemistry , biochemistry , biology , microbiology and biotechnology , receptor , organic chemistry
Calcium release from internal stores of Entamoeba histolytica , a parasitic protozoan, was observed by measuring fluorescence of Fura‐2. Emptying of inositol(1,4,5)trisphosphate (Ins(1,4,5)P 3 )‐sensitive calcium pools in permeabilized E. histolytica did not significantly affect subsequent calcium release by inositol(1,3,4,5)tetrakis‐phosphate (Ins(1,3,4,5)P 4 ). Similarly, prior depletion of Ins(1,3,4,5)P 4 ‐sensitive stores did not have any influence on subsequent calcium release by Ins(1,4,5)P 3 . The EC 50 for calcium release was 0.15 μM with Ins(1,4,5)P 3 and 0.68 μM with Ins(1,3,4,5)P 4 . In conclusion, the Ins(1,3,4,5)P 4 ‐sensitive calcium store in E. histolytica is separate and independent from the Ins(1,4,5)P 3 ‐sensitive pool.