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Novel avidin and streptavidin binding sequences found in synthetic peptide libraries
Author(s) -
Østergaard Soren,
Hansen Per Hertz,
Olsen Marianne,
Holm Arne
Publication year - 1995
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(95)00262-8
Subject(s) - streptavidin , avidin , peptide , biotinylation , peptide library , chemistry , biotin , amino acid , residue (chemistry) , biochemistry , peptide sequence , combinatorial chemistry , gene
Synthetic resin‐bound peptide libraries made of protein l ‐amino acids have been synthesized. During screening of the libraries, peptides that bind to avidin have been identified containing a novel motif with two histidines separated by one residue. A sub‐library was synthesized and screened, and new critical residues appeared surrounding the two histidines. Additionally peptide libraries made of d ‐amino acids have been screened with avidin and streptavidin and novel motifs have been found.