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A new member of the cytosolic O ‐acetylserine(thiol)lyase gene family in Arabidopsis thaliana
Author(s) -
Barroso Consuelo,
Vega JoséM.,
Gotor Cecilia
Publication year - 1995
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(95)00255-8
Subject(s) - arabidopsis thaliana , complementary dna , lyase , arabidopsis , northern blot , southern blot , microbiology and biotechnology , gene , biology , biochemistry , cytosol , chemistry , enzyme , mutant
A cDNA, Atcys‐3A, encoding O ‐acetylserine‐(thiol)lyase has been isolated from Arabidopsis thaliana . The deduced peptide sequence showed a high level of similarity with the bacterial counterpart, and a remarkable percentage of identity with other higher plant O ‐acetylserine(thiol)lyase genes. Sequence comparision and Southern blot analysis suggested that Atcys‐3A was a new and different to the previously reported member of the cytosolic gene family in Arabidopsis . The Atcys‐3A expression was activated by sulfur limitation, requiring a carbon and nitrogen source for maximal expression. A similar pattern of regulation was observed at the O ‐acetylserine(thiol)lyase activity level. Northern blot analysis also showed an organ‐specific expression of Atcys‐3A .