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Sequence specificity for removal of uracil from U·A pairs and U·G mismatches by uracil‐DNA glycosylase from Escherichia coli , and correlation with mutational hotspots
Author(s) -
Nilsen Hilde,
Yazdankhah Siamal Pour,
Eftedal Ingrid,
Krokan Hans E.
Publication year - 1995
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(95)00244-4
Subject(s) - uracil dna glycosylase , deamination , uracil , dna glycosylase , escherichia coli , dna , biology , genetics , lac repressor , gc content , microbiology and biotechnology , dna repair , gene , chemistry , biochemistry , enzyme , genome , lac operon
The rate of removal of uracil from different positions in double‐stranded DNA by uracil‐DNA glycosylase from Escherichia coli varied more than 15‐fold. Consensus sequences for good and poor removal were 5′‐(A/T)UA(A/T)‐3′ and 5′‐(G/C)U(T/G/C)‐3′, respectively. In general, the sequence context surrounding U was more important for the rate of removal than whether U was present in U·A pairs or U·G mispairs. Rates of removal of U from sites of amber mutations in the lacI gene, where mutation frequencies and deamination rates were known, indicated that the observed variation in removal is biologically significant.