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Site‐specific 15 N‐labelling of oligonucleotides for NMR: the trp operator and its interaction with the trp repressor
Author(s) -
Ramesh Vasudevan,
Xu Yao-Zhong,
Roberts Gordon C.K.
Publication year - 1995
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(95)00226-y
Subject(s) - oligonucleotide , labelling , chemistry , repressor , stereochemistry , cytidine , operator (biology) , nuclear magnetic resonance spectroscopy , two dimensional nuclear magnetic resonance spectroscopy , biochemistry , dna , enzyme , gene , transcription factor
A convenient and economical method is described for the site‐specific 15 N‐labelling of the 4‐amino group of individual cytidine residues in oligonucleotides. This is applied to a 20 base‐pair oligonucleotide corresponding to the trp operator; this oligonucleotide and its complex with the E. coli trp repressor are studied by NMR, using 1 H‐ 15 N HMQC and 15 N‐edited 1 H‐ 1 H NOESY experiments.