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In vitro binding of Bacillus thuringiensis var. israelensis individual toxins to midgut cells of Anopheles gambiae larvae (Diptera: Culicidae)
Author(s) -
Ravoahangimalala Olga,
Charles Jean-François
Publication year - 1995
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(95)00220-4
Subject(s) - midgut , bacillus thuringiensis , anopheles gambiae , brush border , biology , microbiology and biotechnology , in vitro , instar , larva , bacillales , bacillaceae , biochemistry , bacteria , botany , immunology , malaria , vesicle , genetics , membrane , bacillus subtilis
Midguts from Anopheles gambiae fourth instars were dissected and processed for immuno‐light microscopy. Cloned insecticidal crystal proteins (ICPs) from Bacillus thuringiensis var. israelensis (Bti) were individually expressed in crystal‐negative strains of Bacillus thuringiensis . Tissue sections of A. gambiae were incubated in vitro with each solubilized and trypsin‐activated ICP. Immunodetection of CryIVA, CryIVB, CryIVD and CytA toxins on sections was performed using purified rabbit IgG directed against Bti ICPs, in combination with an anti‐rabbit IgG/peroxidase. CryIVA, CryIVB, CryIVD and CytA toxins were detected on the apical brush border of midgut cells, in the gastric caecae and posterior stomach. CytA was also detected, to a lesser extent, on microvilli of anterior stomach cells.