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Na + ,K + ‐ATPase expression during the early phase of liver growth after partial hepatectomy
Author(s) -
Martínez-Mas Joan Vicenç,
Peinado-Onsurbe Julia,
Ruiz-Montasell Bonaventura,
Felipe Antonio,
Casado F.Javier,
Pastor-Anglada Marçal
Publication year - 1995
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(95)00217-w
Subject(s) - messenger rna , hepatectomy , atpase , protein subunit , basal (medicine) , microbiology and biotechnology , biology , gene expression , medicine , chemistry , endocrinology , enzyme , biochemistry , gene , surgery , resection , insulin
Na + ,K + ‐ATPase expression has been studied in the early phase of liver growth after partial hepatectomy to ascertain whether its increased activity is due to stable effects, involving de novo synthesis and insertion of pumps into the plasma membrane. Na + ,K + ‐ATPase activity progressively increases after partial hepatectomy, reaching a three‐fold induction above basal values 12 h after surgery. mRNA amounts of both α 1 and β 1 subunits are rapidly increased up to two‐fold for α 1 and nearly three‐fold for β 1 , at 9 and 12 h post‐hepatectomy, respectively. This correlates with increased abundance of both subunit proteins. The results prove that the increase of Na + ,K + ‐ATPase activity correlates with higher expression of both subunit proteins and mRNAs, although the characteristics of the induction suggest that some translational and post‐translational events may be equally involved in the increased activity of the pump.