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The endozepine ODN stimulates polyphosphoinositide metabolism in rat astrocytes
Author(s) -
Patte C.,
Vaudry H.,
Desrues L.,
Gandolfo P.,
Strijdveen I.,
Lamacz M.,
To M.C.
Publication year - 1995
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(95)00209-r
Subject(s) - pertussis toxin , phospholipase c , phosphatidylinositol , inositol , astrocyte , incubation , receptor , phospholipase , biology , metabolism , toxin , biochemistry , chemistry , g protein , microbiology and biotechnology , endocrinology , signal transduction , enzyme , central nervous system
Astrocytes synthesize a series of peptides called endozepines which act as endogenous ligands of benzodiazepine receptors. The present study demonstrates that the endozepine ODN causes a dose‐dependent increase in inositol trisphosphate and a parallel decrease in phosphatidylinositol bisphosphate in cultured rat astrocytes. Pre‐incubation of astrocytes with the phospholipase C inhibitor U 73122 or with pertussis toxin totally blocked polyphosphoinositide metabolism. These data show that, in rat astrocytes, ODN stimulates a phospholipase C coupled to a pertussis toxin‐sensitive G protein.