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Expression and characterization of a B cell growth promoting polypeptide derived from the 12 kDa B cell growth factor gene ( BCGF 1 )
Author(s) -
Kovanen Panu E.,
Virtaneva Kimmo,
Harju Leena,
Kantor Carmela,
Gahmberg Carl G.,
Timonen Tuomo
Publication year - 1995
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(95)00186-d
Subject(s) - recombinant dna , fusion protein , microbiology and biotechnology , biology , cell growth , gene , fusion gene , growth factor , peptide sequence , gene expression , amino acid , biochemistry , receptor
The expression and partial purification of recombinant 12 kDa B cell growth factor are reported. The polypeptide was derived from the genomic sequence of the gene ( BCGF 1 ) which is here shown to be a single copy gene that localizes to human chromosome 16. When expressed as a glutathione S ‐transferase fusion protein in E. coli , the protein appears as a 38 kDa polypeptide in Western blot analysis using a peptide antibody. The purified fusion protein stimulates the proliferation of activated human B cells in a dose‐dependent manner, and the active site resides within the 104 carboxy‐terminal amino acids. The availability of biologically active recombinant 12 kDa B cell growth factor will enable its evaluation in B cell growth regulation, and provides a new means of in vitro culturing of human B lymphocytes.