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Novel modification of ceramide: rat glioma ganglioside GM3 having 3‐ O ‐acetylated sphingenine
Author(s) -
Suetake Keiji,
Tsuchihashi Keiko,
Inaba Kotomi,
Chiba Masahiko,
Ibayashi Yukihiro,
Hashi Kazuo,
Gasa Shinsei
Publication year - 1995
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(95)00182-9
Subject(s) - ceramide , acetylation , ganglioside , glioma , chemistry , biochemistry , stereochemistry , cancer research , biology , apoptosis , gene
A novel O ‐acetylated GM3 containing 3‐ O ‐acetyl 4‐sphingenine was isolated with one having a non‐acetylated base from transplanted rat glioma tissue. The presence and position of the acetyl group were estimated by one‐ and two‐dimensional proton nuclear magnetic resonance, and fast atom bombardmentmass spectrometries. In addition, the O ‐acetyl showed higher immunological activity toward anti‐melanoma antibody in the presence of non‐acetylated GM3 in complement‐dependent liposome lysis than did non‐acetylated or acetylated GM3 alone in the liposome, suggesting enhancement of immunological reactivity of the intact tumor cells by a small amount of O ‐acetyl GM3.