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Primary sequence and post‐transcriptional modification pattern of an unusual mitochondrial tRNA Met from Tetrahymena pyriformis
Author(s) -
Schnare Murray N.,
Greenwood Spencer J.,
Gray Michael W.
Publication year - 1995
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(95)00179-d
Subject(s) - tetrahymena pyriformis , transfer rna , biology , tetrahymena , gene , mitochondrial dna , protein primary structure , nucleic acid sequence , mitochondrion , genetics , microbiology and biotechnology , rna , peptide sequence
In a previous investigation of the rDNA region in Tetrahymena pyriformis mitochondrial DNA, we identified a putative tRNA Met gene [Heinonen et al. (1987) J. Biol. Chem. 262, 2879–2887]. On the basis of Northern hybridization analyses, we suggested that this gene is expressed, even though the resulting tRNA would be unusually small and have an atypical dihydrouridine stem‐loop domain. We report here the complete nucleotide sequence and post‐transcriptional modification pattern of this tRNA Met , confirming its predicted primary structure and supporting the view that this structurally aberrant species functions in translation in T. pyriformis mitochondria.