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Stimulatory effect of unsaturated fatty acids on the level of plasminogen activator inhibitor‐1 mRNA in cultured human endothelial cells
Author(s) -
Karikó Katalin,
Rosenbaum Helga,
Kuo Alice,
Zurier Robert B.,
Barnathan Elliot S.
Publication year - 1995
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(95)00170-e
Subject(s) - messenger rna , plasminogen activator , umbilical vein , plasminogen activator inhibitor 1 , biology , microbiology and biotechnology , fatty acid , activator (genetics) , endothelial stem cell , fatty acid synthesis , biochemistry , gene , endocrinology , in vitro
To determine whether unsaturated fatty acids induce changes in the mRNA level of plasminogen activator inhibitor type‐1 (PAI‐1), Northern analyses were performed on human umbilical vein endothelial cells (HUVEC) and vascular smooth muscle cells that were treated with two common fatty acids. Supplementation of cultured HUVEC with docosahexanoic acid (DHA) or with dihomogamma linolenic acid (DGLA), resulted in a concentration dependent, specific increase of the PAI‐1 transcript levels, which was detectable within 2 h. DHA and DGLA treatment of smooth muscle cells did not result in changes in the PAI‐1 mRNA levels. Homology search of the upstream regulatory region of the PAI‐1 gene sequences identified a consensus nucleotide sequence for a fatty acid‐responsive element. Our results indicate that unsaturated fatty acids selectively increase PAI‐1 mRNA levels in endothelial cells, the primary source of circulating PAI‐1 in vivo.