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Interaction of a synaptobrevin (VAMP)‐syntaxin complex with presynaptic calcium channels
Author(s) -
El Far Oussama,
Charvin Nathalie,
Leveque Christian,
Martin-Moutot Nicole,
Takahashi Masami,
Seagar Michael J.
Publication year - 1995
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(95)00156-4
Subject(s) - synaptobrevin , syntaxin , chemistry , microbiology and biotechnology , calcium , biophysics , synaptic vesicle , biology , biochemistry , exocytosis , membrane , vesicle , organic chemistry
Nerve terminal protein complexes implicated in exocytosis were examined by immuno‐isolation from rat brain synaptosomes. Immunoprecipitation with anti‐syntaxin or anti‐VAMP antibodies revealed a syntaxin‐SNAP25‐VAMP‐synaptotagmin complex. Anti‐VAMP antibodies also trapped a distinct VAMP‐synaptophysin complex. A similar fraction (about 70%) of N‐type calcium channels ([ 125 I]ω conotoxin GVIA receptors), was immunoprecipitated by either anti‐syntaxin or anti‐VAMP antibodies, but not by anti‐synaptophysin antibodies (< 4%). The majority of N‐ but not L‐type calcium channels ([ 3 H]PN200‐110 receptors), appear to be associated with a synaptic vesicle prefusion complex.