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Uptake of iron by symbiosomes and bacteroids from soybean nodules
Author(s) -
Moreau Sophie,
Meyer Jean-Marie,
Puppo Alain
Publication year - 1995
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(95)00155-3
Subject(s) - cytosol , nodule (geology) , biochemistry , internalization , biology , rhizobiaceae , ferrous , chemistry , symbiosis , bacteria , enzyme , cell , paleontology , organic chemistry , genetics
Symbiosomes and bacteroids isolated from soybean nodules are able to take up the iron‐citrate complex. The kinetics are characterized by initial high rates of iron internalization, and ATPase inhibitors significantly lower the uptake. This is consistent with an energy‐dependent process on both membranes, although the involvement of a simultaneous facilitated diffusion can not be completely ruled out. Citrate alone is poorly absorbed by symbiosomes; this uptake is greatly enhanced by addition of iron. Iron‐citrate was found both in the nodule cytosol and in the bacteroids. These results provide the first experimental evidence for the existence, at least in young nodules, of an important iron trafficking system from the plant host cell to the microsymbiont, through the peribacteroid membrane.