Premium
κ‐Opioid receptor‐transfected cell lines: modulation of adenylyl cyclase activity following acute and chronic opioid treatments
Author(s) -
Avidor-Reiss Tomer,
Zippel Renata,
Levy Rivka,
Saya Danielle,
Ezra Vittoria,
Barg Jacob,
Matus-Leibovitch Noa,
Vogel Zvi
Publication year - 1995
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(95)00154-2
Subject(s) - adenylyl cyclase , adcy9 , adcy10 , adcy6 , agonist , medicine , chemistry , endocrinology , κ opioid receptor , opioid , opioid receptor , receptor , camp dependent pathway , pertussis toxin , pharmacology , adenylate cyclase toxin , g protein
The opioid receptors μ, δ and κ have recently been cloned. Here we show that κ‐agonists inhibit adenylyl cyclase activity in Chinese hamster ovary cells stably transfected with rat κ‐opioid receptor cDNA. Chronic exposure of the cells to κ‐agonists did not lead to significant desensitization of the capacity of the agonists to inhibit adenylyl cyclase. On the other hand, withdrawal of the agonist following the chronic treatment led to the phenomenon of supersensitivity (‘overshoot’) of adenylyl cyclase activity. Both the inhibition of adenylyl cyclase activity by the acute opioid treatment and the chronic agonist‐induced supersensitivity are pertussis toxin sensitive, demonstrating involvement of G i /G o proteins in both processes.