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Heterologous desensitization of bombesin‐ and vasopressin‐stimulated phospholipase D activity in Swiss 3T3 fibroblasts
Author(s) -
Briscoe Celia P.,
Wakelam Michael J.O.
Publication year - 1995
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(95)00148-3
Subject(s) - homologous desensitization , desensitization (medicine) , phospholipase d , heterologous , receptor , agonist , phospholipase c , bombesin , g protein , g protein coupled receptor , chemistry , endocrinology , medicine , vasopressin , microbiology and biotechnology , biology , signal transduction , neuropeptide , biochemistry , gene
Bombesin‐ and vasopressin‐stimulated phospholipase D (PLD) activities are rapidly desensitized in 3T3 cells, in addition both agonists are subject to heterologous desensitization. Binding studies showed that homologous desensitization was partly a result of loss of cell surface receptors, whilst heterologous desensitization was independent of receptor changes. Pretreatment with either agonist reduced subsequent GTPγS‐stimulated PLD activity by 50% whereas a pretreatment with GTPγS did not attenuate the response, suggesting that the G‐protein or downstream effector systems were affected by receptor activation resulting in desensitization. The desensitization of receptor‐stimulated PLD activation provides support for the phospholipase functioning in a key signalling pathway.