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Wortmannin inhibits insulin‐stimulated but not contraction‐stimulated glucose transport activity in skeletal muscle
Author(s) -
Lee Abraham D.,
Hansen Polly A.,
Holloszy John O.
Publication year - 1995
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(95)00147-2
Subject(s) - wortmannin , glucose transporter , medicine , endocrinology , skeletal muscle , glucose uptake , insulin , chemistry , phosphatidylinositol , stimulation , contraction (grammar) , kinase , biology , biochemistry
In skeletal muscle, glucose transport is stimulated by insulin, contractions and hypoxia. In this study, we used the phosphatidylinositol 3‐kinase (PI 3‐kinase) inhibitor wortmannin to examine whether (i) PI 3‐kinase activity is necessary for stimulation of glucose transport by insulin in muscle, and (ii) PI 3‐kinase mediates a step in the pathway by which contractions/hypoxia stimulate glucose transport. Wortmannin completely blocked insulin‐ and insulin‐like growth factor‐1‐stimulated glucose transport in muscle. In contrast, wortmannin had no effect on the stimulation of glucose transport by contractions or hypoxia, providing evidence that PI 3‐kinase activity is not involved in the activation of glucose transport by these stimuli.