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Zfy1 encodes a nuclear sequence‐specific DNA binding protein
Author(s) -
P Taylor-Harris,
S Swift,
A Ashworth
Publication year - 1995
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(95)00141-u
Subject(s) - nuclear protein , dna , biology , gene , nuclear localization sequence , microbiology and biotechnology , cell nucleus , nuclear dna , dna binding protein , function (biology) , genetics , transcription factor , mitochondrial dna
Zfy1 is a mouse Y chromosomal gene encoding a zincfinger protein which is thought to have some function during spermatogenesis. Here we show that, when introduced into tissue culture cells, Zfy1 is targeted to the nucleus. Two independent signals are present within the protein for nuclear localization. This nuclear Zfy1 protein is able to bind strongly to DNA‐cellulose and, using site‐selection assays, we have identified specific Zfy1 DNA binding sites. Taken together these results suggest that Zfy1 is a nuclear‐located sequence‐specific DNA binding protein which functions during spermatogenesis.