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Effects of lovastatin on adenylyl cyclase activity and G proteins in GH 4 C 1 cells
Author(s) -
Chiloeches Antonio,
Lasa Marina,
Brihuega Fuensanta,
Montes Agustín,
Toro María J.
Publication year - 1995
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(95)00139-z
Subject(s) - adenylyl cyclase , lovastatin , adcy9 , chemistry , gs alpha subunit , adcy10 , medicine , endocrinology , biochemistry , microbiology and biotechnology , biology , enzyme , cholesterol
We studied the effect of lovastatin, a cholesterol lowering drug, on the basal state of G‐proteins in GH 4 C 1 cells. Our data show that the addition of lovastatin markedly decreased the amount of the α‐subunits of the G s and G i ‐proteins in the plasma membrane. The decrease of αs was correlated with a decrease in adenylyl cyclase activity, and both effects were reverted by the presence of mevalonate. As the attachment of G protein subunits to the membrane is dependent on γ‐subunit prenylation, we assume that the mechanism through which lovastatin exerts its effects on G‐proteins is the lack of mevalonate for the synthesis of prenyl residues. In conclusion, our data indicate that some of the effects of lovastatin are mediated through changes in the basal state of G‐protein in the membrane and consequently on adenylyl cyclase activity.