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Chimeric alpha‐beta oligonucleotides as antisense inhibitors of reverse transcription
Author(s) -
Boiziau Claudine,
Debart Françoise,
Rayner Bernard,
Imbach Jean-Louis,
Toulme Jean-Jacques
Publication year - 1995
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(95)00138-y
Subject(s) - reverse transcriptase , rnase h , chimera (genetics) , microbiology and biotechnology , complementary dna , rnase p , oligonucleotide , rna , murine leukemia virus , beta (programming language) , nucleotide , chemistry , biology , transcription (linguistics) , biochemistry , dna , virology , virus , gene , linguistics , philosophy , computer science , programming language
Alpha‐beta chimeric 17‐mer oligodeoxyribonucleotides containing either 5, 10 or 15 beta nucleotides were synthesized. The stability of the RNA/chimera hybrids was only slightly affected by the alpha stretch and by the alpha‐beta link, as was the affinity of the Moloney Murine Leukemia Virus reverse transcriptase for the duplexes. All chimeras inhibited in vitro cDNA synthesis in a cell‐free system to various extent, via the degradation of the RNA target by RNase H.