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Effects of mutations at position 36 of tRNA Glu on missense and nonsense suppression in Escherichia coli
Author(s) -
Gregory Steven T.,
Dahlberg Albert E.
Publication year - 1995
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(95)00132-s
Subject(s) - transversion , missense mutation , transfer rna , nonsense mutation , suppressor mutation , mutant , biology , nonsense , genetics , mutation , microbiology and biotechnology , escherichia coli , aminoacylation , translation (biology) , rna , gene , messenger rna
Mutations in the anticodon of tRNA Glu (UUC) were isolated or constructed and characterized for their ability to suppress cognate nonsense or missense mutations in vivo. The C36‐to‐A36 transversion mutation was isolated as an ochre and an amber suppressor, while the G36 transversion was selected as a CAG missense suppressor. tRNA Glu suppressors of an AAG missense mutation could not be isolated, and a U36 transition mutation introduced into tRNA Glu in vitro conferred no suppressor phenotype. Over‐expression of glutamyl‐tRNA synthetase did not increase the activity of the U36 mutant tRNA Glu , suggesting a defect at the level of translation rather than at the level of synthetase recognition.