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Molecular cloning and functional expression of a novel potassium channel β‐subunit from human atrium
Author(s) -
Majumder Kumud,
De Biasi Mariella,
Wang Zhiguo,
Wible Barbara A.
Publication year - 1995
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(95)00120-x
Subject(s) - xenopus , protein subunit , potassium channel , cloning (programming) , chemistry , subfamily , molecular cloning , microbiology and biotechnology , biophysics , potassium channel blocker , biology , biochemistry , gene expression , gene , computer science , programming language
We report the cloning and functional expression of a novel K + channel β‐subunit from human atrium, hKvβ3. hKvβ3 is highly homologous to the two β‐subunits cloned from rat brain, Kvβ1 and Kvβ2, but has an essentially unique stretch of 79 N‐terminal residues. Upon expression in Xenopus oocytes, hKvβ3 accelerates the inactivation of co‐injected hKv1.4 currents and induces fast inactivation of non‐inactivating co‐injected hKv1.5 currents. By contrast, hKvβ3 had no effect on hKv1.1, hKv1.2, or hKv2.1 currents. Thus, hKvβ3 represents a third type of K + channel β‐subunit which modulates the kinetics of a unique subset of channels in the Kv1 subfamily.