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Alternative polyadenylation generates three low‐pI α‐amylase mRNAs with differential expression in barley
Author(s) -
Skadsen Ronald W.,
Knauer Nancy S.
Publication year - 1995
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(95)00112-m
Subject(s) - polyadenylation , pi , chemistry , amylase , differential (mechanical device) , messenger rna , microbiology and biotechnology , biochemistry , biology , enzyme , physics , gene , thermodynamics
Specific low‐pI α‐amylase genes from barley ( Hordeum vulgare L.) produced alternative mRNAs with a 17‐base 3′ extension (extension 1) or a 17‐base extension beyond this (extension 2). The extended mRNAs do not arise from splicing of downstream sequences, and not all low‐pI genes contain the extended sequences. All three mRNAs occur in aleurones and shoots, while extension 2 is missing from scutella. Also, the unextended mRNAs predominate in total mRNA, but the extended mRNAs predominate in membrane‐bound polysomes. The extended sequences do not occur in previously characterized α‐amylases, but 16 of 18 bases, mainly in extension 1, are identical with a sequence in the 3′‐UTR of PAPI, a putative inhibitor of α‐amylase. These observations suggest that the extended sequences could play a functional role in α‐amylase expression.