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An actin‐related protein from Dictyostelium discoideum is developmentallyregulated and associated with mitochondria
Author(s) -
Irene Murgia,
Sutherland K. Maciver,
Piero Morandini
Publication year - 1995
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(95)00111-l
Subject(s) - dictyostelium discoideum , dictyostelium , schizosaccharomyces pombe , actin , biology , mitochondrion , caenorhabditis elegans , actin binding protein , microbiology and biotechnology , complementary dna , drosophila melanogaster , cytoskeleton , saccharomyces cerevisiae , genetics , actin cytoskeleton , gene , cell
An actin‐related protein (ACLA) has been identified in the cellular slime mould Dictyostelium discoideum . The complete cDNA sequence indicates that within the actin superfamily it belongs to the ARP3 family of actin‐related proteins together with Arp66B from Drosophila melanogaster, Actin2 from Bos taurus, act2 from Schizosaccharomyces pombe and possibly act2 from Caenorhabditis elegans . The ACLA mRNA is regulated during development, showing a maximum between 2 and 4 h after starvation. The protein has been expressed in E. coli and antibodies raised against it. Immunofluorescence microscopy shows that ACLA protein co‐localises with mitochondria; the protein co‐purifies with Dictyostelium mitochondria.