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Phenotypic features of trehalase mutants in Saccharomyces cerevisiae
Author(s) -
Solomon Nwaka,
Bernd Mechler,
Monika Destruelle,
Helmut Holzer
Publication year - 1995
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(95)00105-i
Subject(s) - trehalase , trehalose , saccharomyces cerevisiae , yeast , mutant , biology , phenotype , biochemistry , gene , heat shock protein , heat shock , microbiology and biotechnology
In the yeast Saccharomyces cerevisiae , some studies have shown that trehalose and its hydrolysis may play an important physiological role during the life cycle of the cell. Recently, other studies demonstrated a close correlation between trehalose levels and tolerance to heat stress, suggesting that trehalose may be a protectant which contributes to thermotolerance. We had reported lack of correlation between trehalose accumulation and increase in thermotolerance under certain conditions, suggesting that trehalose may not mediate thermotolerance [Nwaka, S., et al. (1994) FEBS Lett. 344, 225–228]. Using mutants of the trehalase genes, NTH1 and YBR0106 , we have demonstrated the necessity of these genes in recovery of yeast cells after heat shock, suggesting a role of these genes in thermotolerance (Nwaka, S., Kopp, M., and Holzer, H., submitted for publication). In the present paper, we have analysed the expression of the trehalase genes under heat stress conditions and present genetic evidence for the ‘poor‐heat‐shock‐recovery’ phenotype associated with NTH1 and YBR0106 mutants. Furthermore, we show a growth defect of neutral and acid trehalase‐deficient mutants during transition from glucose to glycerol, which is probably related to the ‘poor‐heat‐shock‐recovery’ phenomenon.